https://gardiner-lab.github.io//Protocols and resources used by the Gardiner Lab 2024-10-28T08:46:29+01:00 Jason Gardiner https://gardiner-lab.github.io// Jekyll © 2024 Jason Gardiner /assets/img/favicons/favicon.ico /assets/img/favicons/favicon-96x96.png 2024-08-13T16:53:00+02:00 2024-08-13T16:53:00+02:00 https://gardiner-lab.github.io//posts/Antibiotics/ Jason

Antibiotic Stock Solution Preparation Protocol 1. Purpose: This protocol outlines the steps to prepare antibiotic stock solutions used for bacterial culture experiments. These solutions can be added to culture media to select for bacteria or plants expressing resistance to specific antibiotics. 2. Materials: * Chemicals: Antibiotics: Ampicillin (50 mg/ml) Basta/Glufos...

2024-08-13T16:53:00+02:00 2024-10-14T15:53:11+02:00 https://gardiner-lab.github.io//posts/Plant-half-MS/ Jason

0.5x MS Media with MES Buffer Protocol 1. Purpose: This protocol outlines the preparation of 0.5x Murashige and Skoog (MS) salts media supplemented with MES buffer, which is suitable for plant tissue culture applications. 2. Materials: * Chemicals: MS Salts powder (includes macro and micronutrients) MES hydrate powder (2-(N-morpholino) ethane sulfonic acid) Potassium hydroxide so...

2024-08-13T16:52:00+02:00 2024-08-13T17:26:48+02:00 https://gardiner-lab.github.io//posts/3M-Sodium-Acetate/ Jason

3M Sodium Acetate (CH3COONa) Preparation Protocol at pH 4.8 1. Purpose: This protocol guides the preparation of a 3M Sodium Acetate (CH3COONa) solution with a pH of 4.8, commonly used in various laboratory applications. 2. Materials: Chemicals: Sodium Acetate Trihydrate (CH3COONa·3H2O) Concentrated Acetic acid (CH3COOH) H2O (Water) Equipment: We...

2024-08-13T16:50:00+02:00 2024-08-13T16:50:00+02:00 https://gardiner-lab.github.io//posts/Tris-HCl-Solution-(pH-7.5)/ Jason

Tris-HCl Solution (pH 7.5) Protocol Created By: Jason Gardiner 1. Purpose: This protocol details the preparation of a 1M Tris-HCl solution buffered to pH 7.5, commonly used in molecular biology techniques. 2. Materials: Chemicals: Tris base (molecular weight: 121.14 g/mol) Concentrated Hydrochloric acid (HCl, approximately 37%) Equipment: Analytical...

2024-08-13T15:50:00+02:00 2024-10-14T15:49:46+02:00 https://gardiner-lab.github.io//posts/Solution-protocol-template/ Jason

Chemical Solution Protocol Template Solution Name: [Enter the name of the solution] Created By: [Enter your name and affiliation] Based on: **1. Purpose: * Briefly describe the purpose of this chemical solution and its intended application. 2. Materials: Chemicals: List all chemicals required for the solution, including: Name Chemical Formula Purity/Grade ...